TABLE 3.

Catabolites formed by BphA from different substituted biphenyls and structurally related compounds and analysis of their TMS derivatives by GC-MS

Substrate	Products
	No.	tr (min)b	Relative yieldc (%)	Prominent ionsd (m/z) (relative abundance [% base peak])	Type of compound
2,2′-Di(NO2)-Ba	1	25.4	100	375 (M+, 40), 360 (3), 270 (51), 242 (5), 146 (8), 73 (100)	2,3-Di(OH)-2′-(NO2)-B
2,2′-Di(OH)-B	1	23.2	10–20	418 (M+, 16), 315 (24), 299 (2), 73 (100)	Tri(OH)-B
	2	23.3	80–90	418 (M+, 13), 403 (3), 315 (51), 299 (4), 73 (100)	2,3,2′-Tri(OH)-B
DBF	1	22.2	2–5	256 (M+, 100), 241 (100), 225 (84), 73 (18)	Mono(OH)-DBF
	2	22.7	5–10	256 (M+, 69), 241 (100), 225 (6), 73 (19)	Mono(OH)-DBF
	3	23.3	10–15	418 (M+, 11), 403 (1), 315 (34), 299 (2), 73 (100)	2,3,2′-Tri(OH)-B
	4	23.5	55–65	346 (M+, 34), 256 (10), 241 (12), 184 (12), 168 (15), 156 (25), 147 (34), 139 (15), 73 (100)	DBF-1,2-DHDe
	5	23.6	10–20	346 (M+, 19), 315 (3), 256 (3), 241 (14), 184 (15), 168 (12), 156 (11), 147 (21), 139 (12), 73 (100)	DBF-3,4-DHDe
DBD	1	24.5	85–95	434 (M+, 12), 419 (2), 331 (35), 315 (3), 73 (100)	2,3,2′-Tri(OH)-DPE
	2	24.9	5–15	362 (M+, 10), 272 (23), 257 (33), 166 (40), 73 (100)	DBD-1,2-DHDe

^aB, biphenyl. 

^bt_r values refer to the BPX5 column (see Materials and Methods). 

^cDeduced from total ion chromatogram peak areas. Minor peaks are neglected. 

^dIons known to be typical for the type of compound given are underlined. 

^eTentative assignment of hydroxylated carbons.