Figure 1.

(a) A scheme showing three steps of sequential OGD applied to living SH-SY5Y cells. Firstly, cells were cultured for 24 h after seeding in 5% CO₂, 95% atmosphere (37 °C) in a DMEM with 4500 mg/ml of glucose (DMEM(+ G)). They refer here as control cells. Next, the medium was exchanged to NBA(-G), and cells were placed in a table CO₂ incubator for 1 h, 3 h, or 12 h at 0.1% O₂ (referred to as OGD conditions and OGD cells). Finally, OGD cells were rinsed with a DMEM(+ G) in the atmosphere of 5% CO₂ and 95% air (reoxygenation conditions, in addition, non-OGD cells were kept in DMEM(+ G)). (b) Phase-contrast image showing the morphology of neuroblastoma SH-SY5Y cells cultured for 24 h in NB(+ G), as it induced differentiation resulting in a neuron-like morphology with numerous, fine protrusions (neurite-like structures). Scale bar 50 µm.