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. 2022 Sep 29;12:16276. doi: 10.1038/s41598-022-20623-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Nanomechanical properties of SH-SY5Y neuroblastoma cells after OGD treatment, quantified by the apparent Young's modulus calculated for the indentation depth of 400 nm (a) and 1200 nm (b). Four groups of cells were compared: control (C, NB(+ G)), OGD cells (OGD 1 h, 3 h, or 12 h, NBA(−G)), re-oxygenated OGD cells (RO 24 h, DMEM(+ G)), and control, non-OGD cells (C 24 h) kept in DMEM(+ G) for the same time as re-oxygenated OGD cells. Box plots represent a median (black line), a mean (solid square), standard deviation (whiskers), and 25% and 75% percentiles (box) from n = 60 cells. Statistical significance: ns not statistically significant (p > 0.05), *p < 0.05, **p < 0.01, ***p < 0.001.