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Expression of HreP and HreP S471-A. E. coli ER2566 carrying plasmid pET-P8/P4-3 (A) or pET-P8/P4-3 S471-A (B) was either not induced (−) or induced (+) by the addition of IPTG. Aliquots of 30 μl of culture were mixed with SDS sample buffer, boiled, and stored until analysis by SDS-PAGE. After affinity purification of the induced proteins, fractions were collected and 30-μl aliquots of fractions 1 to 3 were separated on SDS–10% PAGE gels. M, molecular mass standards (in kilodaltons).
