FIGURE 5.

The fibronectin/fibrinogen complex is upregulated in aneurysms after inhibition of solTNF by XPro1595. (A) Dot plots show the top 10 enriched biological function terms from proteins up- (left) or down-regulated (right) in PPE-induced aneurysms from XPro1595-treated mice (n = 7) vs. vehicle (n = 6) at day 7. Dot size indicates number of proteins found to be regulated by XPro1595 for a given term. The ratio shows the proportion of a functional term covered by proteins regulated by XPro1595, and the dot color indicates the level of significance. (B) Volcano plot of aneurysmal proteins and their differential expression in aneurysms from XPro1595-treated mice vs. vehicle. Horizontal dashed line denotes a 0.05 cut-off p-value, and vertical dashed lines denote a log2(fold-change) of 0.5 in either direction. Fibronectin (FN1), fibrinogen chains FGA, FGB, and FGG and their protein-protein interactions are illustrated by use of the STRING resource (74), showing interactions determined experimentally (pink lines), from curated databases (blue lines), and/or from text-mining (yellow lines) (left upper corner of volcano plot). (C) Fibronectin contents in representative abdominal aortic aneurysmal tissue sections from mice infused with elastase and treated with XPro1595 (20 mg/kg) or vehicle (n = 3–5). Black arrowheads indicate positive stained cells. Proteomics data were analyzed by unpaired t-test for each protein followed by fdr correction for multiple testing. M, media; Ad, adventitia.