FIGURE 1.

X-ray computed tomography across scales. X-ray computed tomography covers a wide range of samples sizes and spatial resolution. Detector size and FOV typically constrain the achievable spatial resolution. MicroCT encompasses FOVs from roughly 0.3–300 mm, delivering spatial resolution in the micron range. In the high throughput IMPC screening (Dickinson et al., 2016), E15.5 embryos were imaged using a lateral FOV of ∼13 mm and an isotropic voxel size of ∼13 µm, yielding a spatial resolution of ∼30 µm in reconstructed image volumes (asterisk).