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Amplification of targeted nucleotide sequences through 18S rRNA PCR assay. 1 and 6: B. bigemina, 2: T. lestoquardi, 3: T. luwenshuni, 4: T. annulata, 5: T. orientalis, 7: B. canis vogeli, 8: B. gibsoni, 9: T. equi, 10: T. ovis, 11: Babesia sp. Leopard and 12: NTC. L-100 bp plus DNA ladder (Thermo Scientific, Lithuania) band size (from lower to upper): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1200, 1500, 2000, 3000 bp
