Fig. 2.

Assessment of analytical sensitivity (A) and specificity (B) of 18S rRNA PCR assay. A Amplification of targeted gene from double-fold serially diluted T. annulata positive sample DNA (1st to 11th dilution; Lane 1st—20 ng, 2nd—10 ng, 3rd—5 ng, 4th—2.5 ng, 5th—1.25 ng, 6th—625 pg, 7th—321.5 pg, 8th—156.25 pg, 9th—78.13 pg, 10th—39.06 pg, 11th—19.5 pg DNA, 12th—NTC). B 18S rRNA PCR on sample positive for 1. Trypanosoma evansi, 2. Hepatozoon canis, 3. Anaplasma marginale, 4. Ehrlichia canis, 5. T. annulata, and 6. haemoparasite negative control DNA. L-100 bp plus DNA ladder (Thermo Scientific, Lithuania) band size (from lower to upper): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1200, 1500, 2000, 3000 bp