Table 2.
In-silico restriction enzyme (RE) analysis of different piroplasms based on 18S rRNA sequence
| Animals | Piroplasms | Amplicon size | Unique RE cuts/position |
|---|---|---|---|
| Cattle & Buffaloes | B. bigemina_MZ573175 | 393 bp |
Ban I 120/ EcoRII 215/ MvaI 217/ ScrFI/217 |
| T. annulata_MZ573172 | 419 bp | NspI 235/ | |
| T. orientalis_MZ573176 | 418 bp |
AccIII 262/ Fok I 278/ HpaII 263/ MnlI 96/ MspI 263/ SnaBI 209/ |
|
| B. bovis_ MH257729* | 378 bp |
Ava I 94/ HinfI 194/ |
|
| Dogs | B. gibsoni_MZ573173 | 405 bp | HinfI 210/ |
| B. vogeli_MZ573177 | 408 bp |
BstEII 77/ BstXI 224/ |
|
| Horses | T. equi_MZ573174 | 421 bp |
DdeI 243/ DraI 82/ MboII 80/ |
| B. caballi_MN629354* | 396 bp | MnlI 98/ | |
| Sheep and Goats | T. ovis_MZ573178 | 424 bp | HinfI 224/ |
| T. luwenshuni_MZ573171 | 422 bp | – | |
| T. lestoquardi_MZ701980 | 421 bp |
NciI 223/ NspI 237/ ScrFI 223/ |
*Previously published GenBank sequences