Fig. 4. Inflammatory cells infiltration in kidney and liver tissues.

After treatment for 14 days, the kidney and liver tissues of rats in all 3 groups were harvested and fixed in 4% paraformaldehyde for at least 24 h at room temperature. Fixed samples were then embedded in paraffin and sliced into 5 mm thick sections. Antibodies against CD68 (surface biomarker of macrophage) and MPO (surface biomarker of neutrophils) were used to reflect macrophage and neutrophils infiltration in the kidney and liver tissues. The yellow color represents a positive staining cell (400×).

MPO, myeloperoxidase.