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. 2022 Sep 13;25(10):105135. doi: 10.1016/j.isci.2022.105135

Figure 4.

Figure 4

Activation of PPARγ in Nudt7+/− liver is responsible for hepatic lipid accumulation

(A) The expression level of Pparg in Nudt7+/+ and Nudt7+/− mouse liver was analyzed using qRT-PCR.

(B) Representative image of PPARγ in 12-month-old Nudt7+/− liver compared to Nudt7+/+ liver (n = 3; Scale bars, 20 μm) and positive staining ratio (n = 6 per group).

(C) The expression levels of PPARγ target genes (Cd36, Fabp4, and Il6) were analyzed by qRT-PCR (n = 3).

(D) Genes related to PPARγ signaling were extracted from the RNA sequencing data (n = 3) and subjected to IPA.

(E) Representative images of BODIPY493/508 staining and positive cell counting (n = 6 per group) in primary cultures of Nudt7+/− hepatocytes in the presence of 10 μM rosiglitazone (Rosi) with pcDNA-Nudt7 for 24 h. Scale bars, 200 μm.

(F) Representative images and intensity of PPARγ in NASH liver (n = 22) compared to normal liver (n = 4) biopsy. Scale bars, 100 μm. ∗p ≤ 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 (Unpaired t-test or onw-way ANOVA).