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. 2022 Sep 13;25(10):105135. doi: 10.1016/j.isci.2022.105135

Figure 6.

Figure 6

Restoration of Nudt7 reduces hepatic lipid accumulation, H3K4me3 of PPARγ, and inflammatory responses

(A) Nudt7+/− mice were tail-vein injected with lentiviruses containing Nudt7 or mock control (CON). Representative images of H&E and ORO staining and immunohistochemistry images of H3K4me3 and PPARγ staining (n = 3). Bar graph of H3K4me3 and PPARγ positive staining intensity was represented as the fold of CON (n = 6).

(B) The levels of serum AST, ALT, FFA, and hepatic cholesterol were analyzed (n = 4).

(C) The expression level of Pparg, Cd36, Fabp4, Il6, and Tnfa was analyzed using qRT-PCR (n = 3).

(D) The expression level of Nudt7, Mll3, Mll4, Ccl2, Ccl4, and Cxcl2 was analyzed using qRT-PCR (n = 3).

(E) Representative images of cell mask orange and PMP70 staining and graphs with adipocyte area per filed and percentage of PMP70-positive cell counting (n = 4; Scale bars, 100 μm). ∗p ≤ 0.05, ∗∗p < 0.01, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 (Unpaired t-test).