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. 2022 Sep 13;25(10):105135. doi: 10.1016/j.isci.2022.105135

Figure 7.

Figure 7

Liraglutide suppresses fatty liver induced by Nudt7 deficiency

(A) Representative images of H&E, NUDT7, PPARγ, and H3K4me3 in mouse liver of HFD-fed Nudt7+/+ mice with liraglutide or equal volume of sterile saline i.p. injected mouse.

(B) Representative image of H&E and BODIPY staining and the number of BODIPY positive cells in HFD-fed Nudt7+/− mice with liraglutide or equal volume of sterile saline i.p. injected mouse. (n = 4; Scale bar, 100 μm).

(C) Hepatic FFA, TG, and cholesterol were analyzed in HFD-fed Nudt7+/− mice liver (n = 6).

(D) Representative images of FABP4, SCD1, PPARγ, and H3K4me3 staining (n = 3; Scale bars, 100 μm).

(E) The expression level of lipogenic, lipid metabolism, and cytokine genes was analyzed using qRT-PCR (n = 3).

(F) Representative image of BODIPY staining and the number of BODIPY positive cells in primary hepatocyte with or without 100 nM liraglutide (n = 6; Scale bars, 100 μm).

(G) The expression level of Nudt7, Pparg, Mll3, Scd1, Fabp4, and Ldlr was analyzed using qRT-PCR (n = 3). ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 (Unpaired t-test).