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. 2022 Sep 29;140(13):1533–1548. doi: 10.1182/blood.2022016033

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Figure 5. — CD180 and MRC1 are highly expressed on AML blasts from some patients but not on CD34 stem/progenitor cells. (A) Normalized protein abundance of CD180 in LFQ data for AML patient bone marrow samples, lineage-depleted bone marrow from healthy donors (Healthy Lin⁻), and CD34-selected bone marrow from healthy donors (Healthy CD34⁺). (B) Normalized RNA abundance of CD180 in AML patient samples and the indicated cell types purified from healthy donor bone marrow samples. In both panels A and B, the letters C, D, and E indicate patient samples selected for flow cytometry as shown in the indicated panels. (C-E) Flow cytometry results with staining for CD180 on both AML cells in the blast gate and CD19⁺ B cells (cells that normally express CD180, as a positive control) in the indicated patient samples. Staining of CD34⁺ cells from healthy donor marrow is also shown. (F) Normalized protein abundance of MRC1 in LFQ data for AML patient bone marrow samples, lineage-depleted bone marrow cells from healthy donors (Healthy Lin⁻), and CD34-enriched bone marrow cells from healthy donors (Healthy CD34⁺). (G) Normalized RNA abundance of MRC1 in AML patient samples, and the indicated cell types from healthy donor bone marrow, are shown. In both panels F and G, the letters H, I, and J indicate patient samples selected for flow cytometry and are shown in the indicated panels. (H-J) Flow cytometry results with staining for MRC on AML cells in the blast gate in the indicated patient samples. Staining of CD34⁺ cells and monocytes (cells that normally express MRC1, as a positive control) is shown from healthy donor marrow.

Figure 5. — CD180 and MRC1 are highly expressed on AML blasts from some patients but not on CD34 stem/progenitor cells. (A) Normalized protein abundance of CD180 in LFQ data for AML patient bone marrow samples, lineage-depleted bone marrow from healthy donors (Healthy Lin⁻), and CD34-selected bone marrow from healthy donors (Healthy CD34⁺). (B) Normalized RNA abundance of CD180 in AML patient samples and the indicated cell types purified from healthy donor bone marrow samples. In both panels A and B, the letters C, D, and E indicate patient samples selected for flow cytometry as shown in the indicated panels. (C-E) Flow cytometry results with staining for CD180 on both AML cells in the blast gate and CD19⁺ B cells (cells that normally express CD180, as a positive control) in the indicated patient samples. Staining of CD34⁺ cells from healthy donor marrow is also shown. (F) Normalized protein abundance of MRC1 in LFQ data for AML patient bone marrow samples, lineage-depleted bone marrow cells from healthy donors (Healthy Lin⁻), and CD34-enriched bone marrow cells from healthy donors (Healthy CD34⁺). (G) Normalized RNA abundance of MRC1 in AML patient samples, and the indicated cell types from healthy donor bone marrow, are shown. In both panels F and G, the letters H, I, and J indicate patient samples selected for flow cytometry and are shown in the indicated panels. (H-J) Flow cytometry results with staining for MRC on AML cells in the blast gate in the indicated patient samples. Staining of CD34⁺ cells and monocytes (cells that normally express MRC1, as a positive control) is shown from healthy donor marrow.