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. 2022 Aug 16;14(5):505–527. doi: 10.4168/aair.2022.14.5.505

Fig. 2. Epithelial-derived IL-17A is essential for IL-24-induced neutrophil migration in vitro. (A) The morphology of human peripheral neutrophils was determined by Diff-Quik staining (1,000×, scale bar = 20 μm). The frequency of living cells (B) and purity (C) of isolated human neutrophils were analyzed by flow cytometry. (D, E) The effect of anti-IL-17A mAbs or isotype control IgG antibody on the neutrophil migration ability of IL-24-stimulated 16-HBE cells in a Transwell coculture chamber (200×, scale bar = 50 μm). Data are presented as the mean ± standard deviation and represent 3 independent experiments.

Fig. 2

IL, interleukin; HBE, human bronchial epithelial; IgG, immunoglobulin G; ns, no significance; mAb, monoclonal antibody.

vs. 16-HBE+Vehicle group, ****P < 0.0001; vs. 16-HBE+IL-24 group, ####P < 0.0001.