Fig. 4. Neutralizing IL-17A during different effector phases displays different capacity to orchestrate Th2 and Th17 responses. (A) BALF levels of IL-4, IL-5, IL-13, and eosinophils attractant CCL11 were quantified by multiplex immunoassays (n = 8–10). (B) BALF levels of IL-17A, IL-17F, IL-6, IL-18, as well as neutrophils attractants CXCL1 and CSF-3 were quantified by multiplex immunoassays or ELISA (n = 8–10). (C) Whole lung tissue expression of the Th2 markers Il4, Il5, Il13, Ccl11 and Clca3 was assessed by quantitative PCR (n = 3). (D) Whole lung tissue expression of the Th17 markers Cxcl1, Cxcl3 and Csf3 as well as Il17a and Il17f, was assessed by quantitative PCR (n = 3).

IL, interleukin; Th, T helper; BALF, bronchoalveolar lavage fluid; ELISA, enzyme-linked immunosorbent assay; PCR, polymerase chain reaction; TDI, toluene diisocyanate.

^*P < 0.05; ^**P < 0.01; ^***P < 0.001.