Fig. 6. Exogenous IL-17A shows distinct roles for TDI-induced Th2 and Th17 responses during different effector phases. (A) BALF levels of IL-4, IL-5, IL-13, and eosinophils attractant CCL11 were quantified by multiplex immunoassays (n = 6–8). (B) BALF levels of IL-17A, IL-17F, IL-6, IL-18, as well as the neutrophils attractants CXCL1 and CSF-3 were quantified by multiplex immunoassays or ELISA (n = 6–8). (C) Whole lung tissue expression of Th2 markers Il4, Il5, Il13, Ccl11 and Clca3 was assessed by quantitative PCR (n = 3). (D) Whole lung tissue expression of the Th17 markers Cxcl1, Cxcl3 and Csf3 as well as Il17a and Il17f was assessed by quantitative PCR (n = 3).

IL, interleukin; TDI, toluene diisocyanate; Th, T helper; BALF, bronchoalveolar lavage fluid; ELISA, enzyme-linked immunosorbent assay; PCR, polymerase chain reaction; PBS, phosphate buffered saline.

^*P < 0.05; ^**P < 0.01; ^***P < 0.001.