Fig. 7. Recombinant IL-17A exerts distinct effects on TDI-induced Th2 and Th17 signatures in vitro. The BEAS-2B cells cultured in DMEM medium supplemented with 10% calf serum were grown to 70%–80% confluent and then treated with TDI (2 mM) + PBS or TDI (2 mM) + IL-17A (100 ng/mL) for 24 hours, and then collected for detecting genes expression by quantitative PCR. (A, B) Expression of the Th2 markers Il4, Il5, Il13, Ccl11 and Ccl24 in human bronchial epithelial cell line was assessed by quantitative PCR (n = 4). (C, D) Expression of Th17 markers Cxcl1, Cxcl3, Csf3 as well as Il17a and Il17f in human bronchial epithelium was assessed by quantitative PCR (n = 4). (E) Expression of the transcription factors Tbet, Gata3 and Rorc in human bronchial epithelial cell line was assessed by quantitative PCR (n = 4).

IL, interleukin; TDI, toluene diisocyanate; Th, T helper; DMEM, Dulbecco’s Modified Eagle Medium; PBS, phosphate buffered saline; PCR, polymerase chain reaction.

^*P < 0.05; ^**P < 0.01; ^***P < 0.001.