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. 2022 Aug 16;14(5):528–548. doi: 10.4168/aair.2022.14.5.528

Table. Primer sequences for quantitative PCR.

Gene Species Forward sequence (5′→3′) Reverse sequence (5′→3′)
β-actin Murine GGCTGTATTCCCCTCCATCG CCAGTTGGTAACAATGCCATGT
Muc5ac Murine CAGGACTCTCTGAAATCGTACCA AAGGCTCGTACCACAGGGA
Il4 Murine ACGAGGTCACAGGAGAAGGGA AGCCCTACAGACGAGCTCACTC
Il5 Murine CTGGCCTCA AACTGGTAATGTAG ATGAGGGGGAGGGAGTATAACTC
Il13 Murine CCTCTGACCCTTAAGGAGCTTAT CGTTGCACAGGGGAGTCT
Il17a Murine GAGAGCTTCATCTGTGTCTCTG GCGCCAAGGGAGTTAAAGAC
Il17f Murine CGTGAAACAGCCATGGTCAAG GGGACAGAAATGCCCTGGTT
Ccl11 Murine TGCTCACGGTCACTTCCTTC CTTGAAGACTATGGCTTTCAGGGTG
Clca3 Murine AGGAAAACCCCAAGCAGTG GCACCGACGAACTTGATTTT
Cxc11 Murine AACCGAAGTCATAGCCACACT CCGTTACTTGGGGACACCTT
Cxcl3 Murine CACCCAGACAGAAGTCATAGCC CCGTTGGGATGGATCGCTTT
Csf3 Murine GTGCTGCTGGAGCAGTTGT TCGGGATCCCCAGAGAGT
Tbet Murine GGACGATCATCTGGGTCACATTGT GCCAGGGAACCGCTTATATG
Gata3 Murine CTACCGGGTTCGGATGTAAGTCG GTTCACACACTCCCTGCCTTCT
Rorc Murine ACAACAGCAGCAAGTGATGG CCTGGATTTATCCCTGCTGA
β-actin Human GAGACCGCGTCCGCC ATCATCATCCATGGTGAGCTGG
Il4 Human CACAGAGCAGAAGAACACAACTG GCGAGTGTCCTTCTCATGGT
Il5 Human TGACTTTTGGAAGGGGAGACC AACATGGATGAACCCCGCTT
Il13 Human ATGGCGCTTTTGTTGACCAC ATTGCAGAGCGGAGCCTTC
Il17a Human TCTCATAGCAGGCACAAACTCA AGCAGTAGCAGTGACACCAAT
Il17f Human TTGGACCGCTGAACTTGTGG AAGTACTTGACCATGGCTGGG
IL1β Human GCTCGCCAGTGAAATGATGG CTGGAAGGAGCACTTCATCTGT
Ccl11 Human ACCACCTGCTGCTTTAACCT CTTGAAGATCACAGCTTTCTGGG
Ccl24 Human CTGTTACCTCCGGGTCCTTT GATGATGTGGTGGGCACAGA
Cxcl1 Human AACCGAAGTCATAGCCACACTC AGGAACAGCCACCAGTGAG
Cxcl3 Human AAGATACTGAACAAGGGGAGCAC TTTTCAGCTCTGGTAAGGGCA
Csf3 Human AGAGCTTCCTGCTCAAGTGC TGGCACACTCACTCACCAG
Tbet Human CTGGATGCGCCAGGAAGTT TGGAGCACAATCATCTGGGTC
Gata3 Human CAGCACAGGCAGGGAGTG AGCCTTCGCTTGGGCTTAAT
Rorc Human AAGAAGACCCACACCTCACAAA GCACCCCTCACAGGTGATAA

PCR, polymerase chain reaction.