Table. Primer sequences for quantitative PCR.
Gene | Species | Forward sequence (5′→3′) | Reverse sequence (5′→3′) |
---|---|---|---|
β-actin | Murine | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
Muc5ac | Murine | CAGGACTCTCTGAAATCGTACCA | AAGGCTCGTACCACAGGGA |
Il4 | Murine | ACGAGGTCACAGGAGAAGGGA | AGCCCTACAGACGAGCTCACTC |
Il5 | Murine | CTGGCCTCA AACTGGTAATGTAG | ATGAGGGGGAGGGAGTATAACTC |
Il13 | Murine | CCTCTGACCCTTAAGGAGCTTAT | CGTTGCACAGGGGAGTCT |
Il17a | Murine | GAGAGCTTCATCTGTGTCTCTG | GCGCCAAGGGAGTTAAAGAC |
Il17f | Murine | CGTGAAACAGCCATGGTCAAG | GGGACAGAAATGCCCTGGTT |
Ccl11 | Murine | TGCTCACGGTCACTTCCTTC | CTTGAAGACTATGGCTTTCAGGGTG |
Clca3 | Murine | AGGAAAACCCCAAGCAGTG | GCACCGACGAACTTGATTTT |
Cxc11 | Murine | AACCGAAGTCATAGCCACACT | CCGTTACTTGGGGACACCTT |
Cxcl3 | Murine | CACCCAGACAGAAGTCATAGCC | CCGTTGGGATGGATCGCTTT |
Csf3 | Murine | GTGCTGCTGGAGCAGTTGT | TCGGGATCCCCAGAGAGT |
Tbet | Murine | GGACGATCATCTGGGTCACATTGT | GCCAGGGAACCGCTTATATG |
Gata3 | Murine | CTACCGGGTTCGGATGTAAGTCG | GTTCACACACTCCCTGCCTTCT |
Rorc | Murine | ACAACAGCAGCAAGTGATGG | CCTGGATTTATCCCTGCTGA |
β-actin | Human | GAGACCGCGTCCGCC | ATCATCATCCATGGTGAGCTGG |
Il4 | Human | CACAGAGCAGAAGAACACAACTG | GCGAGTGTCCTTCTCATGGT |
Il5 | Human | TGACTTTTGGAAGGGGAGACC | AACATGGATGAACCCCGCTT |
Il13 | Human | ATGGCGCTTTTGTTGACCAC | ATTGCAGAGCGGAGCCTTC |
Il17a | Human | TCTCATAGCAGGCACAAACTCA | AGCAGTAGCAGTGACACCAAT |
Il17f | Human | TTGGACCGCTGAACTTGTGG | AAGTACTTGACCATGGCTGGG |
IL1β | Human | GCTCGCCAGTGAAATGATGG | CTGGAAGGAGCACTTCATCTGT |
Ccl11 | Human | ACCACCTGCTGCTTTAACCT | CTTGAAGATCACAGCTTTCTGGG |
Ccl24 | Human | CTGTTACCTCCGGGTCCTTT | GATGATGTGGTGGGCACAGA |
Cxcl1 | Human | AACCGAAGTCATAGCCACACTC | AGGAACAGCCACCAGTGAG |
Cxcl3 | Human | AAGATACTGAACAAGGGGAGCAC | TTTTCAGCTCTGGTAAGGGCA |
Csf3 | Human | AGAGCTTCCTGCTCAAGTGC | TGGCACACTCACTCACCAG |
Tbet | Human | CTGGATGCGCCAGGAAGTT | TGGAGCACAATCATCTGGGTC |
Gata3 | Human | CAGCACAGGCAGGGAGTG | AGCCTTCGCTTGGGCTTAAT |
Rorc | Human | AAGAAGACCCACACCTCACAAA | GCACCCCTCACAGGTGATAA |
PCR, polymerase chain reaction.