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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Mater Today (Kidlington). 2022 Feb 23;56:79–95. doi: 10.1016/j.mattod.2022.01.025

Fig 3.

Fig 3.

GNP inhibit proliferation of CC cocultured with CAF and HUVEC. (A) TAF18, HUVEC, and CP20-EGFP or OV90-EGFP cells were seeded, together or separately, to 96-well plates with the composition and number indicated in the table. Cells were grown in serum-free medium. Cells were treated with 25 μg/ml GNP or PBS 16 h after seeding. Images were taken 7 days after seeding. In the table: CP20=CP20-EGFP, OV90=OV90-EGFP. (B) Cells were trypsinized after imaging with 30 μl trypsin/well, resuspended in 200 μl PBS, and OD488 was read 30 min later. Experiments were performed in sextuplicate and repeated 3 times. *, p<0.05, compared to corresponding control. #, p<0.05, compared to group 1. Scale bar: 100 μm.