Fig 4.

GNP alter growth and morphology of CC cocultured with CAF and HMEC. (A) CP20-EGFP or OV90-EGFP cells, alone or with TAF18 and HMEC cells, were seeded to 96-well plates; 1000 cells/well of each cell type. Cells were grown in serum-free medium. Cells were treated with 25 μg/ml GNP or PBS 16 h after seeding. Images were taken 7 days after seeding. The amplified inserts (in red box) highlight OV90 cell morphology. (B) Cells were trypsinized after imaging with 30 μl trypsin/well, resuspended in 200 μl PBS, and OD488 was read 30 min later. Experiments were performed in sextuplicate and repeated 3 times. *, p<0.05, compared to corresponding control. #, p<0.05, compared to CP20 CON or OV90 CON. Scale bar: 100 μm.