TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5-α | 8 | |
| M. xanthusa | ||
| DK1622 | Wild type | 12 |
| DK2630 | csgA741Ω1519 (Tn5) | 29 |
| DK4292 | Ω4400 (Tn5lac) | 16 |
| DK4294 | Ω4406 (Tn5lac) | 16 |
| DK4296 | Ω4445 (Tn5lac) | 16 |
| DK4300 | sdeK4408::Tn5lac (Ω4408) | 16 |
| DK4323 | sglA1 asgA476 Ω4521 (Tn5lac) | 16 |
| DK4368 | Ω4403 (Tn5lac) | 16 |
| DK4469 | Ω4469 (Tn5lac) | 16 |
| DK4521 | Ω4521 (Tn5lac) | 16 |
| DK5204 | Ω4435 (Tn5lac) | 16 |
| DK5206 | Ω4455 (Tn5lac) | 16 |
| DK5247 | csgA::Tn5-132 (Tetr) Ω4400 (Tn5lac) | 15 |
| DK5279 | Ω4414 (Tn5lac) | 16 |
| DK5287 | csgA::Tn5-132 (Tetr) Ω4414 (Tn5lac) | 15 |
| MS1512 | ΔsdeK1 | This study |
| MS1519 | ΔsdeK1 Ω4400 (Tn5lac) | This study |
| MS1520 | ΔsdeK1 Ω4406 (Tn5lac) | This study |
| MS1522 | ΔsdeK1 Ω4435 (Tn5lac) | This study |
| MS1523 | ΔsdeK1 Ω4414 (Tn5lac) | This study |
| MS1524 | ΔsdeK1 Ω4455 (Tn5lac) | This study |
| MS1526 | ΔsdeK1::pJEF39 tandem duplication; sdeK+ ΔsdeK1 | This study |
| MS1527 | ΔsdeK1::pJEF40 tandem duplication; sdeKH286A ΔsdeK1 | This study |
| Plasmids | ||
| pBluescript SKII | Ampr | Stratagene |
| pBGS18 | Kanr | 33 |
| pTrcHisB | Ampr | Invitrogen |
| psdeK1 | Ampr; pBluescript SKII (cut with XmaI-HindIII) containing 1.6-kb AgeI-HindII fragment with sdeK ORF | This study |
| psdeK2 | Ampr; pTrcHisB (cut with BamHI-HindIII) containing ΩsdeK ORF on a 1.6-kb BamHI-HindIII fragment | This study |
| psdeK2.5 | Ampr; psdeK2 with codon substitution (GCG→CAC) in sdeK ORF so as to produce His-SdeKH286A | This study |
| pJEF39 | Kanr; pBGS18 (cut with SmaI-HindIII) containing sdeK ORF with 1 kb of upstream DNA and 500 bp of downstream DNA on a 3.1-kb StuI-HindIII fragment | This study |
| pJEF40 | Kanr; pJEF39 with codon substitution (GCG→CAC) in sdeK to encode SdeKH286A | This study |
a
All M. xanthus strains are derivatives of DK1622.