TABLE 3.
The sdeK mutant rescues a csgA mutant in codevelopment assaysa
Strain(s) plated for development | Total no. of viable spores/mlb | No. of viable csgA741 spores/ml |
---|---|---|
Wild type | 1.5 × 108 | NAc |
csgA741 | 3.2 × 104 | 3.2 × 104 |
ΔsdeK1 | 4.0 × 103 | NA |
Wild type + csgA741 (1:1) | 9.4 × 107 | 5.8 × 107 |
ΔsdeK1 + csgA741 (1:1) | 1.1 × 108 | 1.1 × 108 |
Spores were collected after 3 days of codevelopment as described in Materials and Methods. Spores were plated on CTT, and germinated cells were patched onto CTT plates containing kanamycin to determine strain derivation, as described in Materials and Methods.
Standard deviations were between 15 and 23%.
NA, not applicable.