narR and flanking regions. The 7-kb region containing narR, part of the narKGHJI region, adaA, and bcrA is shown. Unique EcoRI sites and the BalI site (not unique) used for insertional mutagenesis of narR are shown. The DNA regions to which oligonucleotide primers were designed for use in this work are shown as Pr.1 to Pr.6 and Pr.9 to Pr.12, and the arrows indicate the 5′-to-3′ direction of those primers. The region between narR and narK is expanded, and the consensus FNR binding sites are boxed and in bold.