Figure 18.

(A) Schematic representation of the pathway from protein expression to purification using solubility tags. The “MBP” tag in the graph refers to maltose-binding protein (Mal-bp) defined in the manuscript. Reprinted with permission from ref (414). Copyright 2006 Elsevier. (B) Molecular chaperones provide assistance to folding by lowering free energy barriers and preventing aberrant intermolecular interactions (red), which can lead to various forms of aggregates. Reprinted with permission from ref (467). Copyright 2016 The American Association for the Advancement of Science.