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. 2022 Aug 3;122(18):14085–14179. doi: 10.1021/acs.chemrev.1c00757

Figure 9.

Figure 9

Solubilization attempts on phospholamban. (A) Sequence of canine wild-type phospholamban compared to several redesigned soluble peptides from Engle (PLB-COMP-1, PLB-COMP-2), Engelman (SIM-FULL, ADA-FULL), and DeGrado lab (WSPLB). The differences between WSPLB and phospholamban are shown in red, the differences between SIMM-FULL and WSPLB are shown in blue, and the differences between PLB-COMP-1 (and 2) and SIMM-FULL are shown in green. Reprinted with permission from ref (203). Copyright 2003 John Wiley and Sons. (B) Antiparallel tetrameric helical bundle (left), representing the topology of WSPLB tetrameric peptides, with e–e and g–g contacts labeled, and a parallel pentameric WSPLB (right) with side chain identities. Reprinted with permission from ref (214). Copyright 2005 John Wiley and Sons. (C) X-ray crystal structure of WSPLB (21–52, PDB ID: 1YOD) solved to 1.8 Å (left). A model of full-length pentameric WSPLB shows interactions between Q22(g) and N27(e) in the polar switch (right). Reprinted with permission from ref (215). Copyright 2005 Elsevier.