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Activation of conventional dendritic cells (cDC) coincides with the AAV uptake. Whole blood from healthy human donors was stimulated with AAV-LK03 at 5×1011 vg/mL for 24 hours and stained for intracellular AAV and phenotypic markers. Mean fluorescence intensity (MFI) of CD86 staining is shown for (A) cDC, (B) moDC and (C) pDC. Symbols represent values of each donor. Significance was determined with One-way ANOVA with Tukey’s multiple comparisons test. *** p ≤ 0.001.
