Figure 3.

AAV NAbs enhance uptake of AAV vector into blood phagocytes. Whole blood from healthy human donors was stimulated with AAV-LK03 at 5×10¹¹ vg/mL for 24 hours and stained for intracellular AAV and phenotypic markers. Samples were categorized based on their titers of anti-AAV-LK03 neutralizing antibodies as indicated under x axis. Graphs show percentage of AAV⁺ cells within the indicated cell subtype. Figure 1C shows data from the same set of samples. Bars represent the mean ± SEM and symbols represent the individual values of blood samples. Significance was determined by the Kruskal-Wallis test with Dunn’s multiple comparisons test. *p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001;.