Figure 4.

AAV NAbs enhance anti-AAV pro-inflammatory cytokine response in blood. Cytokine and chemokine secretion from whole blood stimulated with 5×10¹¹ vg/mL of AAV-Spark100 for 24 hours. Samples were categorized based on their titers of anti-AAV-Spark100 neutralizing antibodies as indicated under x axis. Bars represent mean fold-change ± SEM of each analyte’s concentration measured in AAV-stimulated relative to unstimulated blood. Symbols represent the values of each donor. Dashed lines signify a 1.5-fold positivity cutoff. Significance was determined by the Kruskal-Wallis test with Dunn’s multiple comparisons test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.