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Schematic illustration of bioorthogonal-mediated bacterial delivery to enhance probiotics colonization in the gut. (A) DBCO group-modification of probiotics in vitro. (B) Oral administration of probiotics to mice. (C) Azido group-decoration of gut inhabitants by N3-DAA. (D) Bioorthogonal conjugation of delivered probiotics with gut inhabitants via click chemistry. (E) Enhanced adhesion of probiotics in the gut with the occurrence of the bioorthogonal reaction.
