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. 2022 Sep 16;13:951281. doi: 10.3389/fimmu.2022.951281

Figure 3.

Figure 3

The essential amino acids of KS20 peptide for the activation of T cell BDC5.2.9 compared with the SG20 peptide. (A) Amino acid sequence alignment of mouse CGRP and IAPP segments studied here. (B) mAb LD96.24 bound weakly to IAg7-SG20 molecules. The binding between mAb LD96.24 and IAg7-SG20 molecules was measured by ELISA. Results are the means ± SEM of triplicate wells. (C) Viruses encoding WT KS20, WT SG20, KS20 mutants, and SG20 mutants were used to infect mouse ICAM+B7+ SF9 insect cells. KS20△1-5 and non-infected wells were used as controls. 3 days later, these insect cells were used as APCs to stimulate BDC5.2.9 T cells. Results are the means ± SEM of triplicate wells. *P < 0.05, **P < 0.01, ***P < 0.001.