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. Author manuscript; available in PMC: 2023 Oct 1.
Published in final edited form as: FASEB J. 2022 Oct;36(10):e22546. doi: 10.1096/fj.202200135RR

Figure 1. The gut is a contributor to circulating TCA cycle intermediates.

Figure 1.

Relative quantification of TCA cycle metabolites (A) and absolute quantification of succinate (B) in portal versus tail plasma samples from 8-weeks old, male mice after 5 hours food removal. Relative quantification of TCA cycle metabolites (C) and absolute quantification of succinate (D) in portal versus tail plasma samples from ad-lib fed 8-weeks old, male mice on either 8AM or 8PM. Norvaline and D4-sodium succinate were used as internal standards for relative and absolute quantification measurement by GC/MS, respectively (n=6 per group in panel A and B; n=5 per group in panel C and D). * p<0.05; ** p<0.01; *** p<0.001. Analysis in A and B performed via paired student t-test. Analysis in panel C and D performed via two-way ANOVA Bonferroni analysis for post hoc comparisons.