Key Resource Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-sodium channel antibody | Sigma Aldrich | Cat# S8809 |
| anti-NeuN antibody | ProtienTech | Cat# 26975-1-AP |
| RFP-Booster | Chromotek | Cat# rb2AF568-50 |
| anti-ChAT antibody | Invitrogen | Cat# PA5-29653 |
| Virus strains | ||
| Lentivirus pLVX, human sodium channel beta subunit 1 | Vector Builder, custom | Uniprot ID Q07699 |
| Chemicals | ||
| H3-Tamoxifen | Tritech AG (Switzerland) | CAS# 10540-29-1 |
| 4-hydroxytamoxifen | Sigma Aldrich | Cat# T176 |
| Tamoxifen | Sigma Aldrich | Cat# T5648 |
| Triphenylene | Sigma Aldrich | Cat# T82600 |
| Cannabidiol | Sigma Aldrich | Cat# C7515 |
| Carbamazepine | Sigma Aldrich | Cat# C4024 |
| 4-hydroxy-N-desmethyl benzophenone (4OH-ND-BP) | Scheidt Lab | CAS# 110025-28-0 |
| 4-hydroxy benzophenone (4OH-BP) | Scheidt Lab | CAS# 1137-42-4 |
| Experimental models: Cell lines | ||
| HEK-293 cells | American Type Culture Collection | Cat# CRL-1573 |
| CHO-K1 cells | American Type Culture Collection | Cat# CCL-61 |
| MDA-MB-231 cells, triple negative ER receptor | American Type Culture Collection | Cat# HTB-26 |
| Experimental models: Organisms/strains | ||
| C57/B6 mice | Charles River | Cat# eC57BL/6NCrl |
| C57/B6 mice Nav1.8 td-tomato | John N. Wood University College London, London |
NaV1.8-tdTomato, Gautron et al. |
| Recombinant DNA | ||
| Human NaV1.1, ptracer IRES GFP | Vector Builder | Uniprot ID Q99250 |
| Human NaV1.6, ptracer IRES GFP | Vector Builder | Uniprot ID Q9UQD0 |
| Human NaV1.7, ptracer IRES GFP | Vector Builder | Uniprot ID Q15858 |
| Human NaV1.8, ptracer IRES GFP | Vector Builder | Uniprot ID Q9Y5Y9 |
| Human NaV1.9, ptracer IRES GFP | Vector Builder | Uniprot ID Q9UI33 |
| Software and algorithms | ||
| pCLAMP 11 software, electrophysiology acquisition | Molecular Devices | Version 11 |
| ClampFit software, electrophysiology analysis | Molecular Devices | Version 11 |
| IGOR pro 8.1 software, electrophysiology analysis | Wavemetrics | Version 8.1 |
| OrigenPro, statistical analysis | OrigenLab | 2022b |
| Prism, statistical analysis | GraphPad | Version 9 |