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. 2022 Sep 9;13:987151. doi: 10.3389/fimmu.2022.987151

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Copyright © 2022 Van Coillie, Schulz, Bentlage, de Haan, Ye, Geerdes, van Esch, Hafkenscheid, Miller, Narimatsu, Vakhrushev, Yang, Vidarsson and Clausen

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SDS-PAGE of purified recombinant IgG1 and FcγRIIIa-158F/V. Reducing SDS-PAGE of 1 μg purified (A) glycoengineered IgG1 produced in CHO cells with the heavy chain and light chain at ~50 and 30 kDa, respectively. (B) HEK293-expressed His- and AviTag-tagged FcγRIIIa-158F (left lane) and -158V (right lane) migrating as a broad band with a molecular weight ranging from 40 to 50 kDa. Designations for monosaccharides according to the CFG are indicated (51).