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. 2022 Sep 30;53:74. doi: 10.1186/s13567-022-01092-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Deiodinase gene expression. Transcriptional abundance for DIO1 (A), DIO2 (B), and DIO3 (C) in maternal endometrium (END), fetal placenta (PLC), fetal liver (LVR), and fetal kidney (KID) derived from fetuses of Sham inoculated control (CON, n = 10) and PRRSV-2 challenged dams at 21 days post maternal infection. Fetuses from challenged dams were classified based on serum and thymus viral load as being uninfected (UNIF, n = 10), high viral load viable (HV-VIA, n = 10), or high viral load with meconium staining (HV-MEC, n = 10). Gene expression was evaluated using a standard curve generated from plasmid and normalized as copy number per 20 ng of equivalent cDNA to quantify gene expression in each group in each tissue with different superscripts denoting statistical differences between groups (P < 0.05) within a given tissue.