Table 1.

Gene specific primer sequences used for qPCR

Pathway	Official symbol	Gene ID	Forward primer	Reverse primer	Annealing temp (°C)	Amplicon length (bp)	Target RefSeq
Deiondination	DIO1	414380	5′-ACTTCATGCAAGGCAACAGG-3′	5′-GGTCCTGGAGATTCTGGTGA-3′	61	213	NM_001001627.1
	DIO2	414379	5′-CTCGGTCATTCTCCTCAAGC-3′	5′-TCACCTGTTTGTAGGCATCG-3′	61	140	NM_001001626.2
	DIO3	414378	5′-CCTATCTGCGTGTCTGACGA-3′	5′-GCCTGCTTGAAGAAATCCAG-3′	61	92	NM_001001625.2
Sulfation	SULT1A3	396640	5′-GGTGTCCCACAGGTTTTGAG-3′	5′-CGACGTAGACCACCTTGACC-3′	61	123	NM_213765
	SULT1B1	100624541	5′-TGGCTGGAAATGTGGCTTAT-3′	5′-TCACCAGAGGGTTGTCCTTC-3′	61	228	XM_005656511
	SULT1C2	100623541	5′-GAAACCTCAGTCAGCGGAAA-3′	5′-TTCGGGTTCCTCTTGATGTC-3′	61	125	XM_013995894.2
	SULT1E1	397052	5′-GGGAGGAATTTTGGGTGACTA-3′	5′-GCCAGATTTGGGATAGGTGA-3′	61	204	NM_213992.1
	SULT2A1	641359	5′-CCAAGGAAATGTGCCCTATG-3′	5′-GTCCCGTTTCAGCTCTTCAT-3′	61	109	XM_005664644
	STS	448816	5′-AGACCCTCAGGACTCCCAAT-3′	5′-AAACTCCCACCTGGTTCTGA-3′	61	162	XM_021080518
Transportation	SLC16A2	100513513	5′-AGTGGAGTTCCAAGCAGCAT-3′	5′-AGCCCAAACGATCAGTGAAT-3′	61	95	XM_021080455.1
	SLC16A10	100513770	5′-CACCCATTGCAGGGTTACTC-3′	5′-TATGGAGCCAAGGGATGAAA-3′	61	117	XM_021091212.1