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Effect of the virB8 mutations on protein stability. The level of VirB8 and its mutants were monitored by Western blot assays using purified VirB8 antibodies (16). Lanes 1, 2, and 13, uninduced A348, induced A348, and PC1008/pAD1433, respectively; lanes 3 to 12, virB8 mutants 10, 13, 17, 19, 21, 24, 26, virB8S87L, virB8T192M, and virB8R107A, respectively.
