Fig. 8.

miR-146a-5p-modified exosomes reduced the toxicity of neurotoxic astrocytes by targeting Irak1 and Traf6. a, b Representative western blot images and quantitative analysis of C3 and Lcn2 gene expression in mixture(TNFα, IL1β, C1q)-induced astrocytes (neurotoxic astrocytes), Irak1&Traf6 over-expressed neurotoxic astrocytes, ExoOE treated neurotoxic astrocytes and ExoOE treated neurotoxic astrocytes followed by over-expressing Irak1&Traf6. c Representative immunostaining images of GFAP (red)/C3 (green)/Lcn2 (green) and the quantification of the relative immunofluorescence intensity of C3 and Lcn2 in each group. Scale bar = 50 μm. d Representative images and quantitative analysis of neurite length in NGF-stimulated PC12 cells treated with conditioned medium of neurotoxic astrocytes (Mix), Irak1&Traf6 over-expressed neurotoxic astrocytes (OE(Traf6&Irak1)), ExoOE treated neurotoxic astrocytes(ExoOE) and ExoOE treated neurotoxic astrocytes followed by over-expressing Irak1&Traf6(ExoOE + OE(Traf6&Irak1)).Scale bar = 50 μm. e Cell viability of PC12 cells treated with conditioned medium of different groups. Data above are represented as mean ± SD. *, p < 0.05; **, p < 0.01