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. 2022 Sep 28;221(11):e202110132. doi: 10.1083/jcb.202110132

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© 2022 Jani et al.

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Figure S4. — Depletion of PI4KIIs does not alter the neck and width of endosomal nascent tubules. (A) Schematic representation of the measurements (in Fig. 5 D; and Fig. S4, B and D) of the nascent endosomal tubules captured by EM. (B–D) Quantification of the average maximum width (B), length:width ratio (C), and neck width (D) of the endosomal nascent tubules in HeLa cells treated with siCTRL (width, n = 42; length:width, n = 40; neck, n = 30) or siPI4KIIs (width, n = 50; length:width, n = 46; neck, n = 49). Data from three independent experiments are presented as the mean ± SEM (siCTRL, n = 8 cells; siPI4KIIs, n = 14 cells). (B–D) Two-tailed unpaired t test; ns, not-significant. *, P < 0.05.