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BLOC-1 tubulates PI4P-positive GUVs. Spinning-disc confocal microscopy on PI4P+ fluorescent GUV before (left) or after (right) addition of purified BLOC-1 (0.2 µM; see Materials and methods). BLOC-1 addition led to the appearance of multiple tubular structures (arrows). Acquisition parameters: 0.2 s exposure, one z-stack (0.2 µm). Video was shown at 1 frame/s. Scale bar: 5 µm. See also Fig. 2.
