Figure 4.

Effect of treatment of normal wounds with the antiangiogenic pigment epithelium-derived factor (PEDF). Normally, healing wounds go through a phase of capillary dysfunction when the wound bed fills with poorly formed and leaky capillaries. The treatment of wounds with antiangiogenic factors, such as the endogenous antiangiogenic factor PEDF, improves healing and leads to (A) decreased blood vessel leakiness. Photomicrograph and quantification of FITC-dextran leakage from vessels in control versus recombinant PEDF (rPEDF)-treated wounds. Red = CD31, green = dextran. (B) Increased collagen maturity: collagen maturity determined using Picrosirius Red staining. (*) P < 0.05, (**) P < 0.01. (C) Increased pericyte coverage: capillary maturity assessed by % pericyte coverage of microvessels. (**) P < 0.01. (PBS) Phosphate buffered saline. (Figure adapted from Wietecha et al. 2015, courtesy of the authors and The American Physiological Society © 2015.)