TABLE 2.

Effect of ompU and ompT mutations on virulence factor expression and/or secretion and antimicrobial resistance

Relevant genotypea	Autoagglutination	CT secretion (ng/ml of supernatant/OD600)	TCP (CTX φ-Cm transductants/ml/OD600)	Polymyxin B MIC (μg/ml)	Serum resistance (no. of survivors relative to wild type/OD600)	CT-B localization (% total)
						Cells	Medium
Wild type	+	594	3.8 × 105	0.30	1.0	0.3	99.7
ΔompU1	−	17.8	5.9 × 104	0.15	0.97	2.9	97.1
ΔompU2	+	307	1.4 × 105	0.30	0.84	0.3	99.7
ΔompU2 ΔompT	+	104	1.1 × 105	0.30	1.36	1.1	98.9
ΔompT	+	211	1.6 × 105	0.30	1.06	0.6	99.4
ΔtoxR	−	0	0	0.60	0.54	0.1	99.9
ΔtoxR ΔompT	−	0	0	0.30	1.27	1.6	98.4
ΔepsD	+	43.8	2.7 × 105	NDb	ND	70.5	29.5
ΔepsE	+	NDb	1.5 × 105	ND	ND	74.9	25.1

^aThe actual strains used (described in the text) were O395, KKV669, KKV780, KKV884, KKV809, KKV61, KKV804, KKV1216, and KKV1234. 

^bND, not determined.