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. 2022 Sep 30;12:16419. doi: 10.1038/s41598-022-20818-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A–C) Report mean and 95% CI intervals for leukocytes, lymphocytes and CD3+ cells as reported above stratified by timepoint and treatment (MCO vs. high flux dialysis). P values were obtained from linear mixed effect models with subject ID (nested in sequence) with treatment (MCO = 1), period and sequence as main effects. A P values of < 0.05 was considered statistically significant. For CD3+ cells as well as (B) and (C) flow cytometric analysis was performed on re-thawed cryopreserved PBMCs which had been collected before the dialysis session (0 h) at all four study visits. Cells were then either left untreated (B) or stimulated with PMA + Ionomycin (C)—viability was generally > 85%. Means and SD can be obtained from Table 4. For details with regards to the gating strategy see supplement.