Figure 4: C16:1- and C8-AcpP=FabF active site organization.

A) 2D schematic of C16:1-AcpP=FabF active site. The oxyanion hole is not formed and Phe400 is rotated away from the active site. B) 3D rendering of C16:1-AcpP=FabF active site and crosslinker. C) Acyl-binding pocket of C16:1-AcpP=FabF compared to that of C12-FabF (PDB: 2GFY). D) 2D schematic of C8-AcpP=FabF active site. The oxyanion hole is formed and the gating loops are in the closed conformation. E) 3D rendering of C8-AcpP=FabF active site and crosslinker. F) Acyl-binding pocket of C8-AcpP=FabF compared to C12-FabF (PDB: 2GFY). In panels C and F structural alignments were performed over all atoms. (TWO COLUMN)_