Table 1:

Quantification of condensation and transacylation rates of FabF mutants

	C6-AcpP		C12-AcpP		Transacylation
FabF	Rate min−1	% rel. to wt	Rate min−1	% rel. to wt	Rate min−1	% rel. to wt
wt	1.569 ± 0.104	100.00	1.414 ± 0.083	100.00	3.686 ± 0.200	100.00
F400A	0.024 ± 0.006	1.56	0.026 ± 0.004	1.85	0.165 ± 0.010	4.48
G310M	0.027 ± 0.002	1.72	0.026 ± 0.005	1.83	0.014 ± 0.026	3.73
G310F	0.041 ± 0.003	2.59	0.029 ± 0.002	2.08	-	-
D265A	0.072 ± 0.009	4.61	0.059 ± 0.008	4.18	0.018 ± 0.005	0.49
D265N	0.216 ± 0.008	13.76	0.091 ± 0.003	6.41	0.009 ± 0.003	0.24
N404A	0.049 ± 0.025	3.14	0.025 ± 0.002	1.74	0.049 ± 0.011	1.32
G399A	1.284 ± 0.098	81.83	1.622 ± 0.046	114.75	1.643 ± 0.006	44.58
G402A	0.301 ± 0.046	19.18	0.201 ± 0.013	14.18	0.039 ± 0.033	10.65
H268A	0.566 ± 0.039	36.10	0.475 ± 0.013	33.62	0.379 ± 0.014	10.29
M269A	1.771 ± 0.028	112.90	1.897 ± 0.047	134.18	-	-
T270A	0.209 ± 0.012	13.30	0.150 ± 0.013	10.60	0.021 ± 0.005	0.58
S271A	1.085 ± 0.012	69.15	1.153 ± 0.059	81.57	-	-
Y267A/P273A	1.240 ± 0.048	79.01	1.139 ± 0.074	80.53	-	-
Loop Swap	0.010 ± 0.001	0.64	0.008 ± 0.001	0.59	0.003*± 0.001	0.08

^*Estimated rate from total product formation at 50 min.

- Indicates that the assay was not performed.