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Southern hybridization analysis of the chromosome and plasmid detected in strain CA10 cells using the 16S rRNA gene (EcoRI-PstI fragment of pTCA16S) (B), the car gene (EcoRI insert of pUCA1) (C), the cat gene (SalI fragment of pTE11) (D), and the tnpA2 gene (PvuI-HindIII fragment in IS5car2) (E) as probes. A photograph of the ethidium bromide-stained agarose gel is shown in panel A.
