Alignments of TnpA1 and TnpA3 identified from strain CA10 with their homologous transposases. The aligned transposases are contained in ISs classified in the IS5 family (32). The identical and similar amino acid residues in all proteins are indicated by asterisks and dots, respectively. The gaps introduced into the alignment are indicated by hyphens. The critical DDE consensus motif and the well-conserved R (or K) residue 7 amino acids downstream from the E residue in N2, N3, and C1 domains (32) are indicated by the white type. The DDBJ, EMBL, and GenBank nucleotide sequence accession numbers are indicated after the names of transposases and the names of ISs or bacterial strains (in parentheses).