TABLE 1.
Bacterial strains, plasmids, and cosmids used in this study
| Strain, plasmid, or cosmid | Relevant characteristics | Source or reference |
|---|---|---|
| Bacterial strains | ||
| Pseudomonas sp. strain CA10 | Car+a | 45 |
| Pseudomonas sp. strain TE1 | Car−a, AN(C− N+)b | 30 |
| Escherichia coli | ||
| JM109 | recA1 Δ(lac-proAB) endA1 gyrA96 thi-1 hsdR17 relA1 supE44 [F′traD36 proAB lacIqZΔM15] | 48 |
| DH5α | F− φ80d lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK− mK+) deoR thi-1 supE44 λ gyrA96 relA1 | Toyobo |
| XL1-Blue MR | Δ(mcrA)183, Δ(mcrCB-hsdSMR-mrr) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac | Stratagene |
| Plasmids | ||
| pUC18/19 | AprlacZ, pMB9 replicon | 48 |
| pBluescript II KS(+/−) | AprlacZ, pMB9 replicon | Stratagene |
| pBluescript II SK(−) | AprlacZ, pMB9 replicon | Stratagene |
| SuperCos1 | Apr Kmr cos | Stratagene |
| pT7Blue(R) | AprlacZ | Novagen |
| pUCA1 | Apr pUC119 with 6.9-kb EcoRI insert of strain CA10 DNA | 50 |
| pUCA601 | Apr pUC19 with 5.8-kb HindIII insert of strain CA10 DNA | This study |
| pUCA610 | Apr pUC19 with 4.0-kb SalI insert of strain CA10 DNA | This study |
| pBCA616 | Apr pBluescript II SK(−) with 1.0-kb SalI-XbaI insert of strain CA10 DNA | This study |
| pUCA617 | Apr pUC18 with 1.3-kb HincII-SphI insert of strain CA10 DNA | This study |
| pUCA618 | Apr pUC19 with 1.7-kb EcoRV insert of strain CA10 DNA | This study |
| pBCA620 | Apr pBluescript II SK(−) with 9.5-kb EcoRI insert of strain CA10 DNA | This study |
| pBCA711 | Apr pBluescript II SK(−) with 4.4-kb EcoRI insert of strain CA10 DNA | This study |
| pBCA721 | Apr pBluescript II SK(−) with 11.3-kb XhoI insert of strain CA10 DNA | This study |
| pBCA722 | Apr pBluescript II SK(−) with 7.2-kb XhoI-SalI insert of strain CA10 DNA | This study |
| pBCA731 | Apr pBluescript II SK(−) with 7.5-kb EcoRV-ClaI insert of strain CA10 DNA | This study |
| pUCA741 | Apr pUC19 with 8.9-kb SalI insert of strain CA10 DNA | This study |
| pBCA751 | Apr pBluescript II SK(−) with 5.3-kb PstI insert of strain CA10 DNA | This study |
| pTE11 | Apr pUC19 with 10.7-kb EcoRI insert of strain TE1 DNA containing the 7.7-kb Tn5-Mob region | 30 |
| pTCA16S | Apr pT7Blue(R) with 1.5-kb PCR-amplified DNA fragment containing partial 16S rRNA gene of strain CA10 | This study |
| Cosmids | ||
| pSCos701 to pSCos709 | Apr Kmr SuperCos1 with EcoRI insert of strain CA10 DNA | This study |
a
Car+ indicates the ability to grow on CAR as a sole source of carbon, nitrogen, and energy.
b
AN(C− N+) indicates that strain TE1 can grow on anthranilate as a sole nitrogen source but not as a sole carbon source.