TABLE 2.

Proposed functions of the proteins encoded by the genes located in the car gene cluster and its flanking region

ORF (gene name)	Positions in the sequence with accession no. AB047548	Probable function	Calculated molecular mass (Da)	G+C content (%)	Amino acid identity (%)	Homology		Accession no.
						Name(s) of protein(s)	Source
ORF9	26,723–28,426	Unknown (fusion gene)	63,238	55.5	40	AntA	Acinetobacter sp. strain ADP1	AF071556
					27	Xy1X	pDK1 (P. putida strain PaW630)	AF134348
ORF10 (tnpA1)	25,375–26,355	Transposase	36,828	58.9	100	TnpA2, TnpA4	Pseudomonas sp. strain CA10	AB047548
					96	TnpA3	Pseudomonas sp. strain CA10	AB047548
					96	TnpA1	P. stutzeri strain AN10	AF039533
					96	Transposase	IS1384 (P. putida strain H)	AF052751
					94	TnpA2	P. stutzeri strain AN10	AF039534
ORF11	24,489–25,001	Unknown	18,850	49.3
ORF12	23,972–24,463	Unknown	18,038	49.2
ORF13	23,447–23,779	Unknown	12,384	55.0
ORF14	22,567–22,971	Unknown	14,672	65.2
ORF15	20,851–22,485	Unknown	57,008	68.7	13a	VgrG	E. coli strain ECOR-50	AF044506
					13a	VgrG	E. coli strain ec-11	AF044503
ORF16	19,244–18,846	Unknown	14,101	62.6
ORF17	18,644–17,697	Reductase component	33,670	67.9	40	VanB	Pseudomonas sp. strain ATCC 19151	M22077
					34	PobB	Pseudomonas pseudoalcaligenes strain POB310	X78823
ORF18	17,646–16,315	Large (α) subunit of terminal oxygenase component	49,399	63.4	24	TdnA1	pTDNI (P. putida strain UCC22)	D85415
					25	AtdA3	pYA1 (Acinetobacter sp. strain YAA)	D86080
ORF19	16,325–15,792	Small (β) subunit of terminal oxygenase component	20,934	64.8	29	PhnY	Burkholderia sp. strain RP007	AF112137
					26	BnzB	P. putida strain BE-81	P08085
ORF20	15,203–14,394	Short-chain alcohol dehydrogenase	27,878	69.1	33	Short-chain alcohol dehydrogenase	Bacillus thermoleovorans strain B23	AB040809
					33	Hypothetical protein X	Pseudomonas aeruginosa	S39654
ORF21	14,347–13,526	Dienelactone hydrolase	29,901	65.4	36	ORF2	Azospirillum brasilense strain Sp7	Q43914
					33	ClcD	P. aeruginosa strain JB2	AF087482
ORF22	11,659–12,645	Putative regulatory protein	36,411	67.6	30	ORF3	P. aeruginosa strain JB2	AF087482
					17	BphS	Pseudomonas sp. strain IC	AF077670
ORF23	10,639–11,655	Putative regulatory protein	38,853	60.9	27	OxoS	P. putida strain 86	Y12655
					19	ThcR	Rhodococcus sp. strain NI86/21	P43462
ORF24	9,062–10,318	Channel-forming protein	47,299	61.7	39	PhaK	P. putida strain U	AF029714
					35	PhaK-like protein	P. putida strain DOT-T1E	AF031417
ORF25 (tnpA2)	8,809–7,829	Transposase	36,828	58.8	100	TnpA1	Pseudomonas sp. strain CA10	AB047548
ORF26 (antA)	7,487–6,084	Large (α) subunit of terminal oxygenase component of anthranilate 1,2-dioxygenase	53,154	61.1	72	AntA	Acinetobacter sp. strain ADP1	AF071556
					43	XylX	pDK1 (P. putida strain PaW630)	AF134348
ORF27 (antB)	6,081–5,590	Small (β) subunit of terminal oxygenase component of anthranilate 1,2-dioxygenase	19,394	62.8	53	AntB	Acinetobacter sp. strain ADP1	AF071556
					43	BenB	P. putida	AF218267
ORF28 (antC)	5,571–4,543	Reductase component of anthranilate 1,2-dioxygenase	37,984	65.6	54	AntC	Acinetobacter sp. strain ADP1	AF071556
					33	XylZ	pWW0 (P. putida strain mt-2)	P23101
ORF29 (tnpA3)	4,385–3,405	Transposase	36,916	58.8	99	TnpA1	P. stutzeri strain AN10	AF039533
					99	Transposase	IS1384 (P. putida strain H)	AF052751
					96	TnpA1	Pseudomonas sp. strain CA10	AB047548
					94	TnpA2	P. stutzeri strain AN10	AF039534
ORF30	3,245–2,085	Putative ABC transporter substrate binding protein	41,687	63.3	24	HbaE	Rhodopseudomonas palustris strain GCA009	U75364
					15	BraC	P. aeruginosa strain PAO	P21175
ORF31	1,929–214	Putative membrane-spanning protein	60,651	65.6	30	OhbE	P. aeruginosa strain JB2	AF087482
					18	HbaG	R. palustris strain GCA009	U75364
ORF32 (carD)	34,668–35,450	2-Hydroxypenta-2,4-dienoate hydratase	27,458	51.6	74	CumE	Pseudomonas fluorescens strain IP01	D63377
					73	IpbE	pRE4 (P. putida strain RE204)	AF006691
ORF33	35,562–36,059	Unknown	18,625	48.0
ORF34	36,389–37,132	Unknown	27,415	44.4
ORF35 (carF)	37,086–38,033	Acetaldehyde dehydrogenase (acylating)	33,265	48.2	71	DmpF	Pseudomonas sp. strain CF600	S24419
					68	XylQ	pWW0 (P. putida strain mt-2)	S35222
					68	MhpF	E. coli strain K-12	P77580
					67	MhpF	Comamonas testosteroni strain TA441	AB024335
ORF36 (carE)	38,030–39,052	4-Hydroxy-2-oxovalerate aldolase	36,651	50.6	82	MhpE	C. testosteroni strain TA441	AB024335
					83	MhpE	E. coli strain K-12	P51020
ORF37	40,520–39,723	Unknown	29,534	52.3
ORF38 (tnpA4)	41,554–42,534	Transposase	36,828	58.9	100	TnpA1	Pseudomonas sp. strain CA10	AB047548
ORF39	42,747–43,577	C terminus of YkoW-like protein (truncated ORF)	31,201	44.6	38b	Protein slr1305	Synechocystis sp. strain PCC 6803	S76238
					36b	YkoW	Bacillus cereus strain ATCC 14579	AJ243712
ORF40	43,934–44,203	Unknown	10,057	38.5

^aHomology was observed in only about a 100-amino-acid region in the N terminus of the deduced amino acid sequence of ORF15. 

^bHomology was observed in only about a 300-amino-acid region in the C terminus of YkoW or slr1305.