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. 2022 Aug 1;33(8):710–719. doi: 10.5152/tjg.2022.21307

Figure 4.

Figure 4.

The effect of butyrate on the expression of Foxp3 and GPR109a in the ileums. Control: Control group, SAP: SAP group, SAP + butyrate: SAP + butyrate group. (A) The mRNA expression of Foxp3 and GPR109a detected by RT-PCR. Expression of Foxp3: Control group versus SAP group: P < .001, Control group versus SAP + butyrate group: P < .01, SAP group versus SAP + butyrate group: P < .01. Expression of GPR109a:Control group versus SAP group: P < .001, Control group versus SAP + butyrate group: P < .01, SAP group versus SAP + butyrate group: P < .05. (B) Foxp3 and GPR109a level by Western blotting. (C) Foxp3 and GPR109a protein relative to GAPDH level. Expression of Foxp3: Control group versus SAP group: P < .05, Control group versus SAP + butyrate group: P > .05, SAP group versus SAP + utyrate group: P < .05. Expression of GPR109a: Control group versus SAP group: P < .05, Control group versus SAP + butyrate group: P > .05, SAP group versus SAP + butyrate group: P < .05. n = 8 in each group. SAP, severe acute pancreatitis.